v How to Use this Database
The following
information is given for each entry in this database: the reference for the data; the reaction studied; the name of the
enzyme used and its Enzyme Commission number; the method of measurement; the
conditions of measurement (temperature, pH, ionic strength, and the buffer(s)
and cofactor(s) used); the thermodynamic data and an evaluation of it; and,
sometimes, commentary on the data and on any corrections which have been
applied to it. The absence of a piece of information indicates that it was not
found in the cited reference. The
database may be queried in two different ways:
o Using values of information stored in the database |(predefined values)
o User Defined Values |(User Defined Values)
·
Using values of information stored in the database |(predefined values )
Predefined
values for data are contained in the database as several distinct classes of
information (i.e. columns): the
enzyme name, the Enzyme Commission number, the method used in the study, the
buffer, an evaluation of the quality of the data, the pH, the cofactor(s), and
comments. The user may select column
values from the selection boxes using the slider bars associated with each
selection box. One may select values
for more than one column and thus perform a multiple query. If no value (this is always the first data
item in the selection box) is chosen from a particular column during a query,
then all the values from that column will be selected for that search. For each of the columns the user may select
either to display or not to display the values by using the ‘SHOW’ check
boxes. These check boxes control which
columns are displayed following the SUBMIT command. Finally, the output can be sorted according to the column values
by using the ‘SORT ON’ check boxes.
· User Input values |User Defined Values
Values for data are contained in the database as several distinct classes of information (i.e. columns): the enzyme name, the Enzyme Commission number, the method used in the study, the buffer, an evaluation of the quality of the data, the pH, the cofactor(s), and comments. One may query the database with user defined values for a given column by using ||User Defined Values. In the compose query option, one may use AND or OR to specify multiple entry values in a given selection box. While several AND or several OR may be used in a given selection box, one may not use both AND and OR simultaneously. Partial names may also be used in the compose query selection boxes. For each of the columns the user may select either to display or not to display the values by using the ‘SHOW’ check boxes. These check boxes control which columns are displayed following the SUBMIT command. Finally, the output can be sorted according to the column values by using the ‘SORT ON’ check boxes.
A list of possible values for each of the columns (selection boxes) can be obtained by viewing the distinct data items |Show distinct values. All of the values that are contained in a given selection box (i.e. a specified column) for all of the references can be viewed by using |Show all values.
Search the
database using predefined values
Predefined
values for data are contained in the database as several distinct classes of
information (i.e. columns): the
enzyme name, the Enzyme Commission number, the method used in the study, the
buffer, an evaluation of the quality of the data, the pH, the cofactor(s), and
comments. The user may select column
values from the eight selection boxes shown below using the slider bars
associated with each selection box. One
may select values for more than one column and thus perform a multiple
query. If no value (this is always the
first data item in the selection box) is chosen from a particular column during
a query, then all the values from that column will be selected for that
search. For each of the columns the
user may select either to display or not to display the values by using the
‘SHOW’ check boxes. These check boxes
control which columns are displayed following the SUBMIT command. Finally, the output can be sorted according
to the column values by using the ‘SORT ON’ check boxes.
Data values are contained in the database as several distinct classes of information (i.e. columns): the enzyme name, the Enzyme Commission number, the method used in the study, the buffer, an evaluation of the quality of the data, the pH, the cofactor(s), and comments. One may query the database with user defined values for a given column by using Compose Query. In this option, one may use AND or OR to specify multiple entry values in any of the given selection box. While several AND or several OR may be used in a given selection box, one may not use both AND and OR simultaneously. Partial names may also be used in the compose query selection boxes. For each of the columns the user may select either to display or not to display the values by using the ‘SHOW’ check boxes. These check boxes control which columns are displayed following the SUBMIT command. Finally, the output can be sorted according to the column values by using the ‘SORT ON’ check boxes.
This
page permits one to query the database by using the reference as the query
string. You may type any part of the reference, for example the year or the
author's name, as the query input. One may use 'and' or 'or' to join more than
one input values. For example, one may use Kim or Krebs or 1993. One may also
use Krebs and
This option allows the user to view all
values for a specific category of information: the reaction, the enzyme name,
the Enzyme Commission number, the method used in the study, the buffer, an
evaluation of the quality of the data, the pH, the cofactor(s), and comments.
It is also possible to establish a direct
link from other databases to our enzyme thermodynamics database. Specifically, the Thermodynamics of Enzyme‑catalyzed
Reactions database accepts a direct link through one or more of the following
key-words: Reactant, EC, Method,
Enzyme, Buffer, pH, and Cofactor. For example |http://xpdb.nist.gov/enzyme_thermodynamics/enzyme_compose_query.pl?EC=1.1.1.47
displays the data for EC number 1.1.1.47